Pawg pov thawj pom kev tiv thaiv kab mob: Pab txhawb rau kev hloov pauv ntawm phage tsis kam ntawm Pseudomonas aeruginosa raws li CRISPR lub cev tiv thaiv kab mob

Kev tiv thaiv kab mob tiv thaiv kab mob yog teeb meem tseem ceeb hauv kev kho mob, thiab Pseudomonas aeruginosa yog lub hom phiaj muaj peev xwm kis tus kab mob phage tom qab phais tas. Kev tshawb fawb hauv chav kuaj pom pom tias Pseudomonas aeruginosa siv CRISPR-Cas hloov pauv lub cev tiv thaiv kab mob los tsim kev tiv thaiv kab mob rau kab mob bacteriophages thiab lwm yam kab mob DNA, hloov pauv sai hauv ib puag ncig ntuj ib puag ncig thiab tsim cov kab mob tiv thaiv kab mob, yog li txwv kev siv tshuaj kho mob. Cov kev tshawb fawb tsis ntev los no tau qhia tias Quorum Sensing (QS) kev tswj hwm qhov qhia tawm ntawm CRISPR-Cas tiv thaiv kab mob ntawm Pseudomonas aeruginosa. QS system ntawm Pseudomonas aeruginosa muaj LasIR thiab RhlIR, uas tuaj yeem tswj hwm qhov kev qhia ntawm lub cev lub cev xws li CRISPR-Cas lub cev tiv thaiv kab mob, virulence, thiab biofilm. LasIR tswj cov kev qhia ntawm 3-oxo-C12-homoserine lactone (3OC12-HSL) teeb liab qhia, RhlIR tswj kev qhia ntawm C4-homoserine lactone (C4-HSL) teeb liab qhia. Yog li ntawd, cov kws tshawb fawb kwv yees tias kev siv tshuaj kho mob rau pawg pov thawj (Quorum Sensing Inhibitors, QSI) tuaj yeem txwv qhov kev hloov pauv ntawm Pseudomonas aeruginosa raws li CRISPR tiv thaiv kab mob thiab siv lawv rau kev tshawb fawb soj ntsuam.

Hauv txoj kev tshawb fawb no, cov kws tshawb fawb tau siv Baicalein ua QSI los kawm nws cov txiaj ntsig ntawm Pseudomonas aeruginosa CRISPR-based phage (DMS3vir) tsis kam. Nyob rau tib lub sijhawm, QS noob hloov pauv ntawm Pseudomonas aeruginosa tau tsim los kom cov noob tsis tuaj yeem tsim 3OC12-HSL thiab C4-HSL. Los ntawm kev ntxiv 3OC12-HSL thiab C4-HSL, kev hloov pauv ntawm phage tsis kam ntawm Pseudomonas aeruginosa raws li CRISPR-Cas lub cev tiv thaiv kab mob tau tshuaj xyuas. Lub tshuab los kawm txog kev nqus pa thiab tshem tawm qhov muaj peev xwm ntawm Pseudomonas aeruginosa. Ib qho ntxiv, cov kab mob uas nkag siab tau yooj yim (CRISPR-KO) thiab cov kab mob tiv taus kab mob (CRISPR-KO deg hloov pauv) tau siv los tswj hwm los kawm txog kev hloov pauv hauv Pseudomonas aeruginosa lub peev xwm los sib tw nrog phage tom qab QS system raug thaiv.

Hauv pawg tswj hwm, hom tsiaj qus phage tsis kam ntawm Pseudomonas aeruginosa tau poob qis thaum phage ntom ntom nce. Txawm li cas los xij, tsis zoo li qhov kev cia siab uas QSI yuav txo qis kev tiv thaiv ntawm Pseudomonas aeruginosa, thaum 100μM baicalein tau ntxiv, qhov kev tiv thaiv ntawm Pseudomonas aeruginosa rau phage nce. Thaum phage-sensitive kab mob kis tau 104pfu lossis 107pfu phage, lawv qhov kev tiv thaiv nce ntxiv. Ib qho ntxiv, kev tshawb fawb pom tias thaum QS system ntawm Pseudomonas aeruginosa raug txwv, nws nyuaj rau phage kis tus tswv tsev. Thaum QS qhov system raug txwv, kev txheeb ze ntawm Pseudomonas aeruginosa hauv ib puag ncig ntawm 106 pfu phage tau nce ntau. Hauv txoj kev tshawb fawb no, QSI cuam tshuam txog kev qhia ntawm hom kab mob IV fimbriae, ua rau ncua kev lysis ntawm kab lis kev cai kab mob, txo tus nqi ntawm phage adsorption, thiab pab txhawb kev hloov pauv ntawm phage tiv thaiv kab mob CRISPR. Yog li ntawd, QSI txo qhov kev kho mob tshwj xeeb ntawm kev kis kab mob pili-type phage ntawm Pseudomonas aeruginosa.